When we control the level of the steam generator in the nuclear power plants, a swell and shrink arises from many disturbances such as feed water rate, feed water temperature, main steam flow rate, and coolant temperature. If we use the conventional type of PI controller in this system, we will not have stability during controlling at lower power, the removal function of disturbances, and a load follow-up control effectively. In this paper, we study the application of a 2-Degree of Freedom(2-DOF) PID controller to the level control of the steam. generator of nuclear power plants through the simulation and the experimental steam generator. We use the parameters $alpha$, $eta$, $gamma$ of the 2-DOF PID controller for the removal of disturbances and the parameters Kp,Ti,Td of the conventional type of PID controller for controlling setpoint. The back-propagation learning algorithm of neural network is used for tuning the 2-DOF PID controller. We can find satisfactory results of the removal of the disturbances and the tracking function in the change of setpoint through the simulation and experimental steam generator.