Optimum conditions of the cellulose-hydrolysis reaction with mixed enzymes(cellulase extracted from Penicellium funiculosum mixed with $eta$-glucosidase extracted from Almod) were investigated to increase the production of glucose from cellulose. Experimental result showed that optimum conditions fro pH, activity ratio of $eta$-glucosidase to cellulase, concentration of mixed enzymes, concentration of cellulose as a substrate, and temperature range were 4.2, 0.4, 0.8, U/mL, 40 g/L, and 37$pm$3$^circ C$, respectively. In these conditions, quantities of glucose productions by using mixed enzymes were larger than those by using cellulase at optimum conditions.