- 해양세균 Bacillus cereus ASK202가 생산하는 Agarase를 이용한 Agarooligosaccharides의 생산
- ㆍ 저자명
- 김봉조,하순득,임동중,송창문,공재열
- ㆍ 간행물명
- 한국생물공학회지
- ㆍ 권/호정보
- 1998년|13권 5호|pp.524-529 (6 pages)
- ㆍ 발행정보
- 한국생물공학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
An agarase was partially purified from the culture broth of marine bacterium Bacillus cereus ASK202. Optimal pH and temperature of this agarase were found to be 7.0 and 40$^{circ}C$, respectively. The maximum productivity of agarooligosaccharides was obtained from 0.3 %(w/v) agar by using of 1 unit agarase. As the results of TLC and HPLC analysis, these oilgosaccharides consisted of neoagarobiose, neoagarotetraose and neoagarohexaose. Under the optimal reaction conditions, 77.5 %(w/v) neoagarobiose and 6.2 %(w/v) neoagarotetraose were produced from agar and the conversion yield of total agarooligosaccharides was 83.7 %(w/v) after for 2 h reaction at 40$^{circ}C$.