To isolate useful and interesting plant genes in large quantities, random sequencing of cDNA clones from potato leaf library treated with ethylene was performed. Partial sequences fo randomly selected 210 clones with the insert of longer than 500 base pair (bp) as well as poly (A) tail have bee compared with sequences in GeneBank, EMBL and DDBJ nucleic acid databases and fostered 193 expressed sequences tags (ESTs). The 210 cDNA clones identified are related to various aspect of metabolic pathways such as glycolysis, amono acid synthesis, translation mechanism, ribosome synthesis, hormone response, stress response, regulation of gene expression, and signal transduction. Among the 193 ESTs, 12 ESTs (29 cDNA clones) appeared more than once and 181 ESTs appeared once regarded as a solitary group. Out of 210 clones, 29 clones (13.8%) have no similarity to the known nucleotide sequences and could serve as a potentially useful resource for plant moldecular biology referring to particular genes. Nucleotide sequencing to generate more ESTs from ethylene-induced as well as non-induced potato leaf is in progress as well.