The purpose of this study was to investigate effect of iron intakes on utilization of macrominerals (Ca, Mg, Na, K) in rats fed adequate, 2 fold, 4 fold iron for 12 weeks. There were no differences in feed intake, body weight gain, serum and liver levels of macrominerals across iron groups. Ca level in kidney of 4 fold iron group was significantly higher than those in other groups. Excretions of Mg through feces and Ca, Mg, Na, and K through urine were significantly increased with increment of iron intake. In the case of macromineral balances, daily retentions of Mg, Na, and K in adequate iron group were higher than those in 2 /4 fold iron groups. However there was no difference in Ca retention across iron groups. Therefore, it should be considered interaction with macromineral in iron supplementation.