The color difference caused by the overprinting sequence of ink in multicolor printing is due to both trapping and optical properties of ink layers. Hence the effect of optical properties only cannot be analyzed without removing the effect of ink trapping This study was carrier out for the purpose of analyzing optically the color difference caused by only the optical properties of ink under the various printing sequence. The present optical analysis for overprints showed a good agreement with the experimental result. It is expected that this study may contribute to decreasing the color difference between the original and the printed reproduction in multi-color printing.