An experimental study was carried out in a three-dimensional cubic cavity driven by 2-dimensional plane Poiseuille flow for three kinds of Reynolds number, $10^4$, 3 $ imes$ $10^4$ and 5 $ imes$ $10^4$ based on the cavity width and cavity inlet mean flow velcoity. Instant simultaneous velocity vectors at whole field were measured by 2-D PIV system. Laser based illumination and two-frame grey-level cross correlation algorithm are adopted. Severe unsteady flow fluctuation within the cavity are remarkable at above Re = 3 $ imes$ $10^4$ Reynolds numbers and sheared mixing layer phenomena are also found at the region where inlet driving Poiseuille flow is collided with the clock-wise rotating main primary vortex at upper center area. Instant velocity profiles reveal that deformed forced vortex formation is observed throughout the separate two areas.