Habitative microorganisms were isolated from the floated air and surface of record materials of library with ancient archives. The major ecological fungi from them were isolated and identified as Aspergillus niger, Aspergillus oryzae, Neurospora sitophile, Mucor mucedo, Mucor rouxii, Penicilliun notatum, Rhizopus delema, Rhizopus nigricans, Thamnidium elegans, and Tricoderma viridae. When the cellulase activity of fungi isolated from ancient archives and documents was analyzed, Mucor rouxii and Penicilliun notatum showed the highest avicelase and filter paper activity, to 18.089 and 2.819 units, respectively, showing destructive ability of old archives and documents. Whereas, Mucor mucedo revealed the highest CMCase activity of exoglucanase to 7.044 units.