The electrorheological (ER) behavior of chitosan suspension in the silicone oil was investigated. Chitosan suspension showed a typical ER response, Bingham flow behavior upon application of an electric field due to the polarizability of the branched amino group of the chitosan particles. The shear yield stress exhibited a linear dependence on the volume fraction of particles and the squared electric field. On the basis of the experimental results, chitosan suspension has been correlated with the conduction models for ER response and found to be an ER fluid.