Distribution of the spinning apparatus and its fine structure were revealed by the light and electron microscopical observations. The silk glands of the garden spider, Argiope bruennichi, were composed of five kinds of large silk glands (major ampullate glands, minor ampullate glands, tubuliform glands, aggregate glands, fIagelliform glands) and 2 kinds of small silk glands (pyriform glands and aciniform glands). The major ampullate glands were connected with the spigots (large spinning tubes) of the anterior spinnerets, however minor ampullate glands were connected with the middle spinnerets. Among 3 pairs of the tubuliform glands, one pair connected with the spigots on the middle spinnerets and two pairs with the posterior spinnerets. Moreover, sticky capture threads were originated from a characteristic "triad" structure, the arrangement of three spigots made by one flagelliform and two aggregate glands. And it has been also revealed that the pyriform glands on the anterior spinnerets and the aciniform glands on the middle and posterior spinnerets were connected with small spinning tubes (spools) respectively.