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Isolation of a Pseudomonas aeruginosa Strain Capable of Degrading Acrylamide
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  • Isolation of a Pseudomonas aeruginosa Strain Capable of Degrading Acrylamide
  • Isolation of a Pseudomonas aeruginosa Strain Capable of Degrading Acrylamide
저자명
Arvind. Kumar,Kumar. Ashok
간행물명
Journal of microbiology and biotechnology
권/호정보
1998년|8권 4호|pp.347-352 (6 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A new strain of Pseudomonas aeruginosa growing in a rice field contaminated with herbicide and effluents of a factory manufacturing explosives was isolated. This isolate showed excellent growth in unusually high concentration of acrylamide (60 mM). It utilized acrylamide as the sole source of carbon and nitrogen for growth. Other amides such as acetamide, butyramide, isobutyramide, and methacrylamide were also utilized for the growth by this isolate. Acrylamide was degraded into acrylic acid and ammonia by the enzyme amidase. More than $65\%$ of added acrylamide (40 mM) was converted into acrylic acid after 40 h of growth of the culture. Amidase activity was inducible, the highest activity being observed with isobutyramide ($12.5{mu}M$ ammonia/mg protein/min). These results demonstrate that this bacterium can degrade a variety of amides.