Separate type thermosyphon has larger critical heat flux than non-loop type thermosyphon, because the flooding phenomenon of vapor and liquid occurring in non-loop one does not occur. The experimental study has been carried out separate type thermosyphon with single tube. An investigation of heat transfer characteristics in separate type thermosyphon is performed experimentally. Heat transfer coefficients in an evaporator and condenser were measured experimentally. The effects of liquid filling ratio, height difference, cooling temperature and heat flux on the heat transfer coefficients were examined. As a result, the reasonable range of the liquid filling ratio and the dependence of heat transfer on vapor temperature and heat flux are obtained.