This study was carried out to develop the control system with PLC and its operating software and to investigate its control ability of greenhouse environments. Two experimental greenhouses were controlled by PLC and ON/OFF controller, respectively. In greenhouse controlled by PLC, target values of air temperature, relative humidity and $CO_2$ concentration were automatically changed. In warm-water heating, the variation of air temperature was reduced to $pm$ $0.6^{circ}C$ by the method of proportional-integration(PI) control with an inverter. In ventilation, the variation of air temperature was reduced, since windows open and close with multistage by mutual relation formula among the target, indoor, and outdoor temperature. Relative humidity at daytime was maintained with range of 35% to 55% by PLC controlled fogger. $CO_2$ concentration was automatically controlled from 300 to 800 $mu$molㆍ$mol^{-1}$ according to amount of solar radiation. The suppling amount and frequency of nutrient solution were controlled by total integrated solar radiation. Difference in the yield of cucumber in the greenhouse controlled by PLC and by ON/OFF controller was not significant at the 5% level.