- 저장 온도에 따른 Photobacterium phosphoreum의 Viability와 Bioluminescene
- ㆍ 저자명
- 김현숙,정성제,정계훈,전억한,Kim. Hyeon-Suk,Jeong. Seong-Je,Jeong. Gye-Hun,Jeon. Eok-Han
- ㆍ 간행물명
- 한국생물공학회지
- ㆍ 권/호정보
- 1999년|14권 5호|pp.523-527 (5 pages)
- ㆍ 발행정보
- 한국생물공학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
A naturally luminescent bacterium, Photobacterium phosphoreum was stored in 2.5% NaCl solution at 2$0^{circ}C$, 4$^{circ}C$, -2$0^{circ}C$ and -7$0^{circ}C$ for 30 days. In vivo luminescence and concentrations of total and culturable cells were determined by luminometer, spectrophotometer and dilution plate counting, respectively. When stored at 4$^{circ}C$ and 2$0^{circ}C$, concentrations of cells were rapidly decreased as a result of cell lysis, leading to adrop in turbidity and cultured counts. The bioluminescence of cells stored at 4$^{circ}C$ was maintained until 12 days while those of cells starved at other temperatures decreased to background level within 3 days. Following incubation of stored cells in fresh liquid medium, activities of viable cells increased throughout storage period excepting cells stored at 2$0^{circ}C$. Changes in bioluminescence intensity following addition of 2.5% NaCl solution markedly showed in cells stored at -2$0^{circ}C$ and -7$0^{circ}C$ and increased to maximum 8 fold.