To declare conformance of multi-protocol Implementation Under Test(IUT), every layer of the multi-protocol IUT should be tested. According to ISO9646, single-layer test method is applied to testing the highest layer of multi-protocol IUT and single-layer embedded test method is used for the layers by layer all the protocols in a multi-protocol IUT. This paper proposes a new method for testing a multi-protocol IUT. The proposed test method assumes that a multi-protocol IUT is under test and that the interfaces between the layers cannot be controlled or observed by the tester. We apply the proposed test method to TCP/IP and compare the application results with those of the existing test turns out that the proposed test method significantly reduces the number of test cases as well as the number of test events while providing the same test coverage. In addition, the proposed test method shows the capability to locate the layer that is source of failure in testing multi-protocol IUTs.