In this paper, we studied the response characteristics of $alpha$, $eta$ separated type, combined type, PI typed, and feedforward type in 2DOF-PID controller through the simulation and the experiments designed with the multivariable flow control system. The parameters $alpha$ and $eta$ give an affect to characteristics of controller in separated type but $gamma$ does not give an affect to the characteristics of 2-DOF PID. The more $eta$ increases, the more overshoot decreases and especially, in case of PI type represent clearly. The $alpha$, $eta$ separated type has a very small overshoot and its magnitudes in 2-DOF PID onctroller increases in order of $alpha$, $eta$ combined type, PI type, feedforward type, conventional type. The response characteristics of simulation are similar to that of experiments but the experimental characteristics in the multivariable flow control system has the delayed response. The time delay of response in experiments depends on 2-DOF parameter $alpha$, $eta$, $gamma$ and the overshoot increase as the $alpha$, $eta$, $gamma$ increase. So, we can have a satisfactory response by tuning D gain.