Recently structural damage has been frequently observed in reinforced concrete brdiges due to repeated loads such as vehicular traffic an due to continual overloads by heavy duty trucks. Therefore, the purpose of this experimental stduy is to investigate the damage mechanism due to fatigue behavior of high-strength reinforced concrete beams under repeated loads. From the test results, the relation of cycle loading to deflection is on the mid-span , the crack growth and the modes of failure according to cycle number, fatigue life and S-N curve were observed through the fatigue test. Based on the fatigue test results , high-strength reinforced concrete beams failed to 57 ∼66 percent of the static ultimate strength . Fatigue strength aobut two million cycles from S-N curves was certified by 60 percent of static ultimate strength.