This study was carried out to investigate the effect of Pine needle extract on lipid oxidation and free radical reaction in iron sources reacted with active oxygen species. The results were summarized as follow; The pine needle extracts didn`t show a distinct effect on reduction of lipid oxidation if the iron ion didn`t exist in oil emulsion. The pine needle extracts played role as a strong chelating agents to bind iron ion if Ferrous iron(Feulcorner) exist in oil emulsion. Ferric iron(Fe) was lower effect than Ferrous iron(Fe) on free radical reaction in oil emulsion. And also, the Feulcorner reacted with pine needle extract did not show distinct effect on free radical reaction, compared to Feulcorner reacted with pine needle extract. And also, Pine needle extracts reacted with HulcornerOulcorner were tended to show a low oxygen scavenging ability in case of HulcornerOulcorner only was existed, compared to those of HulcornerOulcorner + Feulcorner complex. Pine needle extracts were the most powerful Feulcorner binding agents, compared to other strong synthetic antioxidants such as EDTA and DTPA.