Male mice (ddY strain) were fed with the chow diet containing 10% sucrose supplemented with orotic acid at the 1% level or/and taurine at the 5% levels for 14 days. The concentrations of triacylglycerol and cholesterol in the liver were significantly lower in the orotic acid group than the control group. When taurine and orotic acid were administered simultaneously, the concentrations of triacylglycerol and cholesterol in the liver were higher and lower, respectively, compared to the orotic acid group. The concentration of triacylglycerol in the serum was higher in the taurine group than that of the control or the orotic acid groups, and the simultaneous supplementation of orotic acid and taurine further enhanced. There were no significantly difference in body weight gain, diary food intake, and the concentrations of serum cholesterol and hepatic phospholipid. These results suggest that dietary taurine stimulated the increasion of hepatic triacylglycerol by orotic acid in mouse.