Carrot (Daucus carota L.) suspension cells exhibit a number of physiological responses when carbon sources in the medium are depleted (i.e., carbon-source starvation). We previously reported that activities of several phospholipid catabolic enzymes, such as phopholipase D (PLD) and lipolytic acyl hydrolase (LAH), are induced to provide cells with alternative carbon sources. In this study we report sequence of PLD cDNA. When starvation was prolonged over approximately five days, cells started to die. To analyze the initiation of cell death, we examined the degradation of DNA and activity of DNA endonuclease. Preliminary results showed that DNA degradation occurred at the onset of cell death. Our findings suggest that carrot cells exhibit two different phases-acclimation response and cell death-during starvation. In working toward a long-term objective of understanding the whole scope of biochemical events during starvation, we have also catalogued the genes induced by starvation.