Response surface methodology(RSM) was applied to determine the optimum heating conditions(tem-perature and time) for the inactivation of bacteria associated with food hygiene. Coefficients of determinations(R2) for the cell growth were 0.9155(P<0.05) in Pseudomonas aeruginosa 0.8997(P<0.05) in Escherichia coli 0.9690(P<0.01) in Salmonella typhimurium 0.9393(P<0.05) in Enterobacter aerogenes and 0.7905 in Vibrio parahaemolyticus. The growth of strains was significantly affected by the temperature and time and reaction to temperature was most closely correlated to the inhibition of growth. On the basis of superimposed contour maps of cell growth the optimum range of heating conditions were 55.62-6$0^{circ}C$ and 19.25-30 min.