This experiment was to investigate decoloization characteristics of E1 effluents from the bleaching plant of pulp mill with three white-rot fungi(Trametes versicolor, Ganoderma appanatum and Pleurotus ostreatus).In addition, the effect of carbon and nitrogen resources was discussed on its decolorization. The color removal of E1 effluent during shaking and stationary cultures were 72% and 80%, respectively. Stationary culture was more effective on decolorization of E1 effluent compared to the shaking culture. The optimum inoculum weight was 1.0g based on dry weight of mycelia . The decolorization medium I showed 88% of the color removal of E1 effluent with in one day cultivation of T.versicolor and P.ostreatus . Color removal was increased from 87% to 90%. T.versicolor and P.ostreatus by the addition of 0.5% glucose. By addition of nitorgen sources(ammonium sulfate and ammonium choride), medium was much higher than that of carbon source. With 0.1% ammoniumm sulfate, P.ostreatus and T.versicolor showed 94% and 92% of the color removal within one day of cultivation , respectively. On decolorization medium II, T.versicolor and P.ostretus were 94% of oclor removal with addition of carbon source. The addition of nitrogen source was much more efficient than that of carbon source. With 0.1% amminium chloride, T.versicolor and P.ostreatus showed 95% of its color removal . The decolorization medium II was higher color removal than medium I, and also MnP and laccase were produced. However, the decolorization medium I produced a little MnP and laccase activity. It could be suggested that MnP and laccase may play an important role in decolorization of E1 effluent.