Antibiotic activity of selected biocontrol agent Trichoderma harzianum ABGS-95 showed 59% to P. graminicola, 65% to P. aphanidermatum and 57% to Rhizoctonia solani compare to non-treated control. ABGC-95 showed resistant to major agrochemicals such as metalaxyl+mancozeb, etridiazole, propamocarb, toclofos methly, terbuconazole, pencycuron and flutolanil. The biocontrol agent T. harzianum ABGC-95 grew vigorously in low nutrient media and water agar. And sand mixture with wheat bran or mowing debris of zoysia grass also provided good growth of the organism. Application of sand mixture of Trichoderma spp. into aeration cores in golf showed most effective biocontrol of pythium blight. Top dressing application of T. harzianum ABGC-95 reached 83% control efficient while spray application of same biocontrol agent showed only 69% control. The biocontrol agent ABGC-95 successfully suppressed the population density of Pythium spp. in soil. The population density of total Pythium spp. in ABGC-95 treated soil was sustained almost same population at beginning(early May) up to end of August, while the population in untreated control plot was increased 5 times that of beginning and even 10 times in pathogen accumulated soil.