The accurate estimation of segmental duration is crucial for natural-sounding text-to-speech synthesis. For predicting Korean segmental durations, conventional methods utilized phonemic context, part-of-speech context and locational information in prosodic phrase. In this paper, the tonal information of phonemes is employed for more accurate prediction. After defining two non-boundary tones and six boundary tones, we annotated the tonal label on each syllable of 400 sentences. To predict segmental duration using tonal information, we constructed neural networks with a real-valued output node predicting phonemic duration and trained them by backpropagation algorithm. Experimental results showed that the proposed features are effective for predicting Korean segmental durations, and we got 0.863 correlation coefficient of the observed durations and predicted ones.