Phylogenic relationships among the six species (D. testacea, D. sternopleuralis, D. bizonata, D. hisrrio, D. immigrans, D. curviceps) of the subgenus Drosophila in Korea were investigated by taximetrical analysis, allozyme and soluble proteins analysis. The taximetrical analysis of the 6 species according to the 30 morphological characters were performed using UPGMA. Two species D. bizonata and D. testacea clustered at high similarity enough to suggest that they were closely related species. A cladogram was constructed by cladistic analysis among 6 species with 30 morphological characters. These species were divided into two lines at their ancestors level: D. histrio, D. bizonata and D. testacea belonged to the one line, while D. immigrans, D. curviceps and D. sternopleuralis belonged to the other. The genetic relationships among the six species in the subgenus Drosophila species group were investigated following that eighteen loci were detected out of analysis fifteen allozymes by means of starch gel electrophoresis. Fum, Ao, Acph, Xdh, Me, Odh-2, G6pd and Sdh had monomorphic patterns in the each species, and specially Fum, Acph, Xdh, Sdh was same allele. The average genetic similarity of the six species which was belong to subgenus Drosophila in Korea was 0.531. Soluble proteins were analyzed by TDE. In genetic distance by TDE, D. immigrans and D. curviceps are even closer than any other ones showing genetic distance 0.264, D. bizonata and D. testacea are also closely related species. The results of taxonomical analysis, allozyme and soluble proteins analysis were similar. These results might suggest that the subgenus Drosophila consist of four group; the 1st group of D. immigrans, D. curviceps, 2nd group of D. bizonata, D. testacea, 3rd group of D. sternopleuralis group and 4th group of D. histrio group.