The vortex tube is a sample device for separating a compressed gaseous fluid stream into two flows of high and low temperature without any chemical reactions. The phenomena of energy separation through the vortex tube were investigated experimentally, to see the effects of the number of nozzle holes on the energy separation. The experiment was carried out with the number of nozzle holes from 1 to 10 by varying inlet pressure and cold mass fraction. The experimental results were indicated that the effective number of nozzle holes for the best cooling performance was found as 4. Also, to find effective use in a given operation conditions, the temperature difference of cold air and the cooling capacity of vortex tube was compared. The result is that cooling capacity was more important than temperature difference of cold air.