Blood chemical values and sex steroid hormone concentration of repeat breeder and reproductive disorder in Hanwoo, which were bred in Chunchon areas, were examine to establish the basic physiological maker. The concentrations of albumin, blood urea nitrogen(BUN), calcium(Ca), cholesterol, creatinine, glucose, phosphorous, total protein and triglycerides in repeat breeder and reproductive disorder were 3.28$pm$0.05 and 3.27$pm$0.04 g/㎗, 11.07$pm$0.61 and 12.69$pm$0.88 mg/㎗, 9.98$pm$0.10 and 9.56$pm$0.11mg/㎗, 105.75$pm$3.57 and 126.78$pm$5.66mg/㎗, 1.62$pm$0.05 and 1.65$pm$0.09 mg/㎗, 67.46$pm$9.07 and 76.97$pm$3.76mg/㎗, 6.13$pm$0.36 and 6.11$pm$0.26mg/㎗, 5.82$pm$0.08 and 6.18$pm$0.08 g/㎗ , 12.82$pm$1.46 and 15.19$pm$1.61mg/㎗, respectively. Among the blood chemical values, Ca, cholesterol and inorganic phosphorous levels in repeat breeder and reproductive disorder were slightly higher than those in normal cows. The progesterone levels of pregnancy(0.18~6.56 ng/$mell$) and non-pregnancy(0.15~5.46ng/$mell$) cows at estrus cycle were showed similar trends from 0 day to 18 days. Those of pregnancy cows were increased gradually from 18 days to 180 days(5.90~7.51 ng/$mell$), while those of non-pregnancy cows were dramatically drop at 21 days (0.18$pm$0.03mg/㎗). The concentrations of estrogen and progesterone in repeat breeder and reproductive disorder were 57.42$pm$5.03, 56.76$pm$8.73 pg/$mell$ and 6.26$pm$0.83, 9.41$pm$1.85 ng/$mell$ . respectively. These results indicate that blood chemical values in repeat breeder and reproductive disorder were not greatly difference in normal cows except for Ca, cholesterol, BUN and inorganic phosphorous. Since the progesterone levels in repeat breeder and reproductive disorder was maintained to the luteal phase levels of normal cows, it may cause of failing of conception and next estrus cycle.