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Rapid Somatic Embryogenesis and Plant Regeneration in American Ginseng: Effete of Auxins and Explants
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  • Rapid Somatic Embryogenesis and Plant Regeneration in American Ginseng: Effete of Auxins and Explants
  • Rapid Somatic Embryogenesis and Plant Regeneration in American Ginseng: Effete of Auxins and Explants
저자명
Wang. X.,Proctor. J.T.A.,KrishnaRaj. S.,Saxena. P.K.,Sullivan. J.A.
간행물명
Journal of ginseng research
권/호정보
1999년|23권 3호|pp.148-163 (16 pages)
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고려인삼학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

The efficacy of three auxins, viz. 2,4-0, NAA and dicamba, were compared for the induction of somatic embryogenesis in American ginseng (Panax quinquefolium L.). Somatic embryos (SEs) formed on ginseng cotyledonary, zygotic embryo and shoot explants after 8 weeks of induction by the auxin stimuli. Significantly more somatic embryos were induced by culture of any of the ginseng explants on media supplemented with $5{mu}M$ 2,4-0 than any other auxin treatment. Shoots derived from somatic embryos had the greatest regenerative potential and zygotic embryos the least. Explants generated from green (unstratified) seeds gave similar or higher frequency of embryogenesis as the explants derived from stratified seeds. Histological and SEM studies confirmed that the regenerimts were somatic embryos. Somatic embryos germinated and developed into normal plants in $3~6$ months. About $10\%$ of plantlets from second generation SEs formed flowers within 10 weeks, particularly on media supplemented with $GA_3$ The development of a regeneration system for ginseng through somatic embryogenesis is a necessary first step for mass propagation and genetic improvement of American ginseng.