The concentrations of 4 kalosaponins from tissues of Kalopanax septemlobus (Thunb.)Koidz grown in 7 provenances in Korea were determined by HPLC. Kalosaponin contents in plant part were much higher in the inner bark(30.59 mg/g on the dry weight basis) than those of young leaves(22.74 mg/g on the dry weight basis) and root bark(18.02 mg/g on the dry weight basis). A considerable range of variation in the contents was observed among population. The kalosaponin contents in inner bark from each population were highest in the Mt. Barwang (30.37 mg/g on the dry weight basis) followed by Mt. Gariwang, Hanra II, Mangun, Paltan, and Hanra I population. A variation of kalosaponin contents among population may be affected by both environmental and genetic factors. Establishment of selection and propagation of high kalosaponin containing trees can be a good source for the development of valuable forest products.