Introduction : In spite of the use of Bee Venom aqua-acupuncture in the clinics, the scientific evaluation on effects is not enough. Bee Venom aqua-acupuncture is used according to the stimulation of acupuncture point and the chemical effects of Bee Venom. The aims of this study is to investigate the analgegic effects of the Bee Venom aqua-acupuncture, through the change of writhing reflex Materials and Methods : Pain animal model was used acetic acid method. The changes of writhing reflex of the mice which were derived pain by injecting acetic acid into the abdomen, after stimulating Bee Venom aqua-acupuncture on Chungwan($CV_{12}$) and non acupuncture point on the backside were measured. Results : 1. It showed that the writhing reflex were appeared on the groups which injected aceticacid only, and saline-acetic acid group(sample I), but not on the group bee venom-saline group(sample II). 2. The change of writhing reflex by Chungwan($CV_{12}$) Bee Venom aqua-acupuncture showed significant decrease in the order of Chungwan($CV_{12}$) Bee Venom aqua-acupuncture group III($2.5{ imes}10^{-3}$g/kg), II($2.5{ imes}10^{-4}$g/kg), and I($2.5{ imes}10^{-5}$g/kg), compared with control group. There were significant decrease of number of writhing reflex in $5{sim}10,;10{sim}15;and;15{sim}20$ minutes intervals of Chung wan($CV_{12}$) Bee Venom aqua-acupuncture group I, and in $0{sim}5,;5{sim}10,;10{sim}15;and;15{sim}20$ minutes intervals of II and III, compared with control group. 3. The change of writhing reflex by non acupuncture point Bee Venom aqua-acupuncture showed significant decrease in the $0{sim}5;and;5{sim}10$ minutes intervals and the total number of writhing reflex in $2.5{ imes}10^{-4}$g/kg group, compared with control group 4. The effects of writhing reflex of Chungwan($CV_{12}$) Bee Venom aqua-acupuncture group showed significant decrease, compared with non acupuncture point Bee Venom aqua-acupuncture group. Conclusion : This study shows that the Bee Venom aqua-acupuncture on Chungwan($CV_{12}$) decreases the numbers of writhing reflex. As the analgegic effects of Bee Venom aqua-acupuncture is recognized. Bee Venom aqua-acupuncture treatment is expected for pain modulation. In order to use it in many ways, more researches are needed for the dose and stability of Bee Venom aqua-acupuncture.