In order to investigate the effect of the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu($BL_{23}$) and Taejo($BL_{11}$) loci on the estrogen-deficiency osteoporosis induced by ovariectomy in rats, serum osteocalcin estradiol progesterone follicular stimulating hormone(FSH) calcium(Ca) and phosphorous(P) levels were monitored. The results were as follows : 1. Serum osteocalcin levels were significantly (p<0.01, p<0.05) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 2. Serum estradiol levels were increased In the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group, but Taejo group did not show significant value. 3. Serum progesterone levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 4. Serum FSH levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 5. Serum Ca levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu group compared with control group, but Taejo group did not show significant value. 6. Serum P levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. According to these results, the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo loci have favorable effect on treatment and prevention of osteoporosis induced by ovariectomy in rats.