This study was performed to evaluate the effect of Kami-bun-sim-ki-eum on irritable bowel syndrome and to find the factors valuable to diagnosis of symptom-complex of them. Four major sympoms were classified in all 31 paients(female 17, male 14), which was index of abdominal, fecal, autonomic dysfunction, and gastric dysfucton and pain severity score method was used in monitoring with the effect of Kamibun-sim-ki-eum on irritable bowel syndrome before and after adminstration of 2 packs I day for 10 days. We have found that Kami-bun-sim-ki-eum was effective only in female group ,but not in male and only sex factor was recognized significantly among factors to have influenced the effect of Kami-bun-sim-ki-eum. Rate of improvement was mean 61% in female, accompanied with improvement of each symptom after administration. Therefore, we have concluded that Kami-bun-sim-ki-eum can be applyed as one of an choice medicines in treating female irritable bowel syndrone.