P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the $sigma$54-Dependent Promoter, dctA, and Its Transcriptional Regulators

P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the $sigma$54-Dependent Promoter, dctA, and Its Transcriptional Regulators
P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the $sigma$54-Dependent Promoter, dctA, and Its Transcriptional Regulators

ㆍ 저자명: Kim. Euhgbin,Kim. Daeyou,Lee. Joon-Haeng
ㆍ 간행물명: The journal of microbiology
ㆍ 권/호정보: 2000년|38권 3호|pp.176-179 (4 pages)
ㆍ 발행정보: 한국미생물학회
ㆍ 파일정보: 정기간행물|ENG|
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ㆍ 주제분야: 기타

이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.

서지반출

기타언어초록

A challenge phage system was used to study the DNA-protein interaction between C4-dicarboxylic acid transport protein D(DCTD) or $sigma$54, and a $sigma$54 -dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or $sigma$54 directed this challenge phage towards lysogency, indicating that DCTD or E$sigma$54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD(or $sigma$54) and the dctA promoter region.

키워드

P22-based challenge phage $sigma$54 dctA C4-dicarboxylic acid transport protein D(DCTD)

다운URL