This paper describes experimental investigations of helium-air exchange flow through openings with vertical partitions. Such exchange flows may occur following rupture accident of stand pipe in high temperature gas cooled reactor. Exchange flow rates are investigated experimentally by using partitioned opening and opening with extended partition to assess fluids interference of the exchange flow at the stand pipe rupture accident. A tests vessel with the two types of opening on top of test cylinder is used in the experiments. An estimation method of mass increment is developed and applied to measure the exchange flow rate. A technique of flow visualization by Mach-Zehnder interferometer is provided to recognize the exchange flows. Amplitude and progress of interference fringes of the flows are observed and used as a support in comparison with the exchange flow rates. Flow passages of upward flow of the helium and downward flow of the air for both two types of the opening are separated by inserted partition within the opening, but in the case of partitioned opening, unseparated flow is formed at the opening entrance and the two flows interface. The exchange flow rate for the partitioned opening is not greater than that of the opening with extended partition because of the fluids interference at the entrance of opening. Finally, the fluids interference at the opening entrance is found to be one of important factors on the helium-air exchange flow rate.