The cometaboic biodegradation conditionso f trichloroethylene(TCE) by Burkholderia cepacia G4 were optimized using response surface analysis. The experimental sets of phenol concentration temperature and pH were designed using central composite experimental design. The optimal conditions of phenol concentration temperature and pH were determined to be 0.91 ppm 21.5$^{circ}C$ and 7.65 respectively by the Ridge analysis of the contour plot for TCE biodegradation rates. The TCE biodegradation rate could be enhanced up to 2.43 nmol.mg protein$.$min by response surface methodology.