This work is method that uses a hydrolysis for increasing yield of paclitaxel in plant cell cultures. The best pH is 3.0 to obtain a maximum yield at fixed reaction temperature and time t pH 3.0 reaction temperature 80$^{circ}C$ and reaction time 8 hr give the highest yield which is three time of control. This is very simple and efficient method to increase paclitaxel yield in plant cell cultures.