To measure the fuel consumption rate of a vehicle, a car is tested on chassis dynamometer following given driving mode. But the fuel consumption rate measured by this method may be somewhat different from that measured in on-road driving conditions. It may be due to not considering road grade in driving modes. In this study, new driving modes which include road grade are proposed, and the simulation program to estimate the real driving fuel consumption rate of a vehicle is developed. On-road car tests to verify the simulation program are carried out and the results of the simulation are analysed and compared with those of the experiments.