The purpose of this research was to device thermal comfort model for radiation power plant workers in protective clothing. Three fabrics commonly used in protective workwear were made into coveralls of identical design and were evaluated by adult healthy males in four simulated work environment. It was investigated between the physiological response and subjects comfort according to environmental variance and clothing types. The of simulated work enviro mensent was controlled under four different humidity and temperature of each type. (Temperature 20±1℃, RH 40∼70%±5%, Temperature 30±1℃, RH 40∼70%±5%) An index of physiological response was connected with the thermal comfort designed. Mean skin temperature, skim temperature, Axillasy temperature ear canal temperature, clothing climate, total sweat, blood pressure, and R-R interval were be evaluated. Skin temperature difference ocurring during exercise and rest were significant only with respect to time and regions of the body, This despite physical differences in the three coveralls, particulary mass statistically experiment. Also, an index of subject wearing sensation was designed for thermal comfort after investigation determined the kind of clothes and the type of environment. As a result of this research, two types of multiple regressions was deviced to estimate thermal comfort of the protective clothing.