The effects of the simulated acid rain on the growth response and water status of Glycine soja was observed in 2 months-old seedlings. The seedlings were treated with acid rain(pH 3.5), three times per week. Glycine soja showed significant reduction in the total length, total weight, dry weight, water contents and top/root ratio after treatment of simulated acid rain(pH 3.5) for 56 days. The growth of shoot was more susceptible to acid rain than that of roots. On the contray, Number of lateral roots, water saturation deficit and epidermis transpiration was higher than that of control(pH 5.6) after 56 days treatment of simulated acid rain.