This study was conducted to investigate the effect o nutritional states of the males on the growth and the production of proteins in male accessory gland substances(MAGs) and transfer of MAGs proteins to female Aedes alboptictus mosquitoes during mating. A male can mate a maximum of 4 times in a day. There was a significant difference in between the male accessory gland size fed 10% sugar of between the unmated male with a mean gland width of 138${mu}{ extrm}{m}$ and the mated male of 63${mu}{ extrm}{m}$. The total protein content of MAGs from the males was significantly lower than those from the sugar-fed males. Male nutrition, therefore, affected the production of male accessory proteins. The difference in the amount of protein in MAGs between the unmated with 0.245 $mu extrm{g}$/gland and mated MAGs with 0.057 $mu extrm{g}$/gland represented the amount of protein transferred to the female during mating. A significantly greater amount of protein(0.188 $mu extrm{g}$/gland) was transferred from the male to females during mating. With these results, it can be confirmed that adequate nutrition played a role in successful insemination of female because well-fed males transfer more MAG protein to females than starved male of A. albopictus. Two polypeptides of 46.2 KD and 28 KD were shown in unmated spermatecae, suggesting that those two polypeptides might be served as a male factor transferred from male to female in A. albopictus during copulation.