In this study, the fatigue life assessments of steel railway bridge in Japan National Railway were carried out. This railway bridge has been under in service from 1957, and fatigue damages were occurred at bead toe of upper part of vertical stiffeners of stringers, bead toes of scallop in lower part of vertical stiffeners in stringers and crossings of cross beams and stringers. From this study, a series of field tests were carried out by stress history measurement about above mentioned points. And as a results of stress histogram analysis, cummulative fatigue damage rate and fatigue life of these members should be calculate quantitatively. And from this estimation technique, cummulative fatigue damage rate and fatigue life of this railway bridge were strongly affected in passing tonage every year and the histories of live load.