To obtain the genetic information about DVT( $alpha$- and $eta$-4,8,13- duvatriene-1,3-diols) and cis-abienol contents, two burley(Burley 21, KB 108), three sun-cured cultivars(N. tabacum L. cv. Yonbyun 3, Jahengyon and Jaraehong), TI 1068, and thirty F$_1$ hybrids derived from them were sampled and the diterpenes were analysed using thin layer chromatography (TLC) procedures. DVT exudation from the leaf scerface could Ie detected in all coltivars and F$_1$ hybrids tested. TI 1068 and Yonbyun 3 had cis-abienol exdudates. Burley 21, KB 108, Jaheungyon, and Jaraehong had no spot of cis-abienol. It is considered that cis-abienol exdudation might be controlled by dominant gene(s). The cytoplasmic effect on the cis-abienol exdudation was not detected.