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한우에서의 Rotavirus의 분리와 Serotype 결정 및 염기서열 분석
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  • 한우에서의 Rotavirus의 분리와 Serotype 결정 및 염기서열 분석
  • Isolation, Serotyping and Nucleotide Sequence Analysis of Bovine Rotavirus Isolated from Korean Native Cattle
저자명
유제현,차광종,김응률,김유성,이영건,송진욱,조홍찬,주지선,박범석,유대환,김세민,지병주,이중복,Yu. Jae-Hyeun,Cha. Kwang-Jong,Kim. Eung-Ryool,Kim. You-Seong,Lee. Young-Kun,
간행물명
대한바이러스학회지
권/호정보
2000년|30권 3호|pp.189-202 (14 pages)
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대한바이러스학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

This study was conducted to see what types of bovine rotaviruses were isolated at Jedong farm in Jeju province and Seohwa farm in Chungnum province. The results were as follows. 1. Rotavirus was positively detected in 18 out of 39 fecal samples from calves with diarrhea in Jeju province and in 13 out of 18 fecal samples from calves with diarrhea in Chungnam province. 2. The electropherotype pattern of dsRNA for 31 viruses was shown to be 4 : 2 : 3 : 2 type like traditional group A and the imigration pattern of dsRNA was the long type like NCDV (G6), JBR (G6), B223 (G10) and KK3 (G10). 3. The serotypes of the 18 viruses of Jedong and 9 viruses of Seowha were shown to be group A, subgroup I, G6, and P1 by ELISA and PCR analyses. The serotypes of S-2, S-6, S-9 and S-12 viruses of Seowha were shown to be group A, subgroup I, G10, but was not shown to be P type. 4. The partial nucleotide sequence of VP4 of S-8 was 97% homology with that of BRV 033. VP4 of J-10 showed 96% homology with that of BRV 033 in nucleotide sequence.