This study concerns the performance of the heat transfer of the thermosyphon having 80 internal groove in which boiling and condensation occur. Distilled water has been used as a working fluid. The liquid filling as the ratio of working fluid volume to total volume of thermosyphon has been used as the experimental parameters. The heat flux and heat transfer coefficient at the condenser are estimated from the experimental results. The experimental results have been assessed and compared with the existing theories. As a result of the experimental investigation, the maximum heat flow rate in the thermosyphon is proved to be dependent upon the liquid fill quantity. relatively high rates of heat transfer have been achieved operating in the thermosyphon with the internal groove. Also, a thermosyphon with the internal groove can be used to achieve some inexpensive and compact heat exchangers in low temperature. In addition, overall heat transfer coefficients and the characteristics as an operating temperature are obtained for the practical applications.