This study has developed a new liquid crystal calibration technique using Neural networks with median filtering and applied this technique to heat transfer measurements. To verify the validity of this new measurement technique, the local Nusselt numbers on a flat plate surface subjected to an axisymmetric impinging jet were measured and compared with the results by the conventional Hue-temperature calibration technique under the same conditions. Because the Neural networks predict the non-linear relations between temperatures and corresponding R, G, B values, Neural networks-median filtering calibration technique can utilize a much wider color band in the experiment than the Hue-temperature calibration technique, resulting in a significant reduction in the experimental time.