국내외에서 분양 받고 국내식육에서 분리된 총 100균주의 Y. enterocolitica에 대한 병원성여부를 HEp-2세포 침투성 시험법을 기준으로 하고 여러 가지 병원성 확인 방법을 비교하였다. 혈청형만으로 병원성을 판단하기에는 충분하지 않았고 이외의 방법 즉, esculin과 salicin시험, pyrazinamidase 시험, biotype 등의 생화학적 특성에 바탕을 둔 실험 단독으로 병원성을 판단하기에는 완전하지 않았다. D-xylose 발효실험, CRMOX agar 시험과 자동응고반응(Autoagglutination)들은 병원성확인에 보조적인 역할만을 할 수 있었다. HEp-2 세포 침투성 시험법은 시간과 노력이 많이 소요되므로 이 방법을 대치할 수 있는 간단하고 신속, 정확하게 Y. enterocolitica 병원성을 확인할 수 있는 방법은 esculin시험에서 음성반응을 보이는 것만 PCR시험을 행하여 판별하는 것이라고 사려된다.
The pathogenicity for one hundred strains of domestic and foreign Y. enterocolitica was tested with HEp-2 cell invasion method as a reference. The serotyping, biotyping, PCR and esculin hydrolyis, salicin fermentation, pyrazinamidase activity, indole production, xylose fermentation, CRMOX and autoagglutination were compared to determine the possibility of pathogenic detection method. According to the test results, serotyping was limited to verify pathogenicity, however, biotyping was quite related to pathogenicity up to 99%. The biotype 1A strains were non-pathogenic, while all strains of biotype $1B{sim}4$ showed pathogenicity with the exception of one strain belonged to type 1B. The esculin and salicin test results were completely close and correlated to pathogenicity up to 99%. The HEp-2 cell invasion and pyrazinamidase test were related to pathogenicity by 95%. Biochemical tests such as D-xylose fermentation, CRMOX agar test and autoagglutination in broth were effective as a support test. It is strongly recommended that sequencial esculin test and PCR test could be done to verify pathogenicity of Y. enterocolitica as the easiest and accurate procedure.
