Object : Hepatitis Treatment-tang No.1 has been used for the treatment of Liver disease and Jaundice. Long-term EtOH exposure leads to immunoregulatory and detoxification impairment. This study aimed to determine the relationship between TNF-${alpha}$ production and expression, and EtOH-induced cytotoxicity on Hep G2 cells. Method : Cells were incubated with EtOH in the presence or absence of HT. The cells were tested after 24 hours and, again, after 48 hours. Cytoviability and TNF-${alpha}$ release were analyzed by MTT assay and enzyme linked immunosorbent assay (ELISA), respectively. After 24 hours of EtOH exposure, the cytoviability decreased, and the release of TNF-${alpha}$ was increased. Increased amounts of TNF-${alpha}$ contribute to EtOH-induced cytotoxicity. The Anti-TNF-${alpha}$ antibody almost abolished it. Interestingly, EtOH-induced cytotoxicity and TNF-${alpha}$ production were inhibited by HT. Moreover, when HT was used in combination with the anti-TNF-${alpha}$ antibody, there was a marked inhibition of EtOH-induced cytotoxicity. Results : These results suggest that HT may prevent the cytotoxicity through partial inhibition of the TNF-${alpha}$ secretion.