An isolate of the prospective hyperparasite, Ampelomyces quisqualis 94013 (AQ94013) was selected for the use of biological control of cucumber powdery mildew caused by Sphaerotheca fuliginea. Examination for the parasitism processes by scanning electron microscopy and light microscopy showed that conidia of AQ49013 germinated on conidia, conidiophores and hyphae of Sphaerotheca fuliginea four hours after inoculation. Appressorium-like structures were developed and attached to the hyphae of S. fuliginea seventeen hours after inoculation. Hyphae of AQ94013 penetrated into hyphae of S. fuliginea twenty-four hours after inoculation. Pycnidia of AQ94013 were produced in the hyphae and the basal part of conidiophores of S. fuliginea fourty four hours after inoculation. The pycnidia of AQ94013 matured foully eight hours after inoculation, and the conidia were discharged from the ostioles of the pycnidia fifty two hours after the inoculation. At the same time, hyphae and conidiophores of S. fuliginea were distorted and died. Also, concentrated culture filtrate and culture filtrate of AQ94013 had not suppressed the cucumber powdery mildew fungus as water treatment. Therefore, mode of action of AQ94013 was assumed to be parasitism on powdery mildew fungi.