The purpose of this research is to analyze of biotope structure focused on the evaluation for the species and biotope preservation in the case of Soosung District in Daegu metropolitan city. We analyzed the structure of urban biotope, and investigated the disposition of space and several characteristics through the material collection relating to the existing urban ecological material and the field investigation. The results of this study were as follows; 1) The result of biotope type classification was divided into 17 biotope type groups and 90 biotope types belonging to them. 2) The biotope type of mixed forest(QD) has the highest rate in occupation area, 1.764ha and the biotope type of cultivation areas by vinyle-hothouse(KC) has the lowest rate, 0.3ha. 3) In the result of the first evaluation for the species and biotope preservation, biotope types such as QB, QI, PA, PB, NB and NC etc. possess great value. Particulary, these biotope types come from forest areas and transition zone. They have not appeared in the inner of cities. 4) The biotope types such as CA, NA, NB, ND and PB are proved to possess high value in the result of rarity and riskiness evaluation. There appear EE, MA, NB, QC and QD in IV grade which is impossible to have them regenerative. They usually have appeared in forest areas. 5) There emerge 3a biotope type that has the highest value in the result of the second evaluation such as CC, EE, KF, LD, MB, NC, PB, QB and QI etc. Most of them were presented into the large area of site size and were distributed in the forest areas intensively. So, it is certain that the space for the species and biotope preservation connected with residential district is scarce. 6) The detailed biotope plan must be made out specially continuously. It is about biotope spaces that are especially important for the species and biotope preservation from the result of this research. Also, the study on the detailed index settlement of the urban landscape plan based on the biotope map must be continued.