The purpose of this study is to construct the evaluation criteria based on the relative importance and various combination of each evaluation index and then to evaluate urban biotope in terms of conservation of species and habitat. After the standard of evaluation had been developed through the literature review, the relative importance of the evaluation index through expert survey was found out, and the evaluation model of biotope value has been established. The results of this study are as follows; 1) We have constructed the nineteen evaluation criteria through literature reviews, and have provided relative importance to each evaluation index of the diversity of typical species in biotope, afforestation rate, stratification, Hemeroby grads, and the diversity of landscape patterns based on the results of expert survey. and then we have constructed the evaluation system using the relative importance criteria. 2) To evluate urban biotope using the evaluation system constructed, we selected 14 sites. The final evaluation results of each site is as follows; The III grade areas are Seohan and Hwasung high-rise apartment at Beummul-Dong, Korea Men Power /Agency in Sungs 대 industrial zone, the adjacent area of St. Tongil, and Hyomyung elementary school. The II grade areas are the farm land behind the third Anshim Jugong apartment, and Ehyun park. The I grade areas are swampy land in front of Kangchon and Anshim construction site, water space with semi-natural style in front of Gangchon Woobang apartment, forest zone near page Temple at mt. Palgong, the edge area of Jisan and Gosan, and the tendril yard against Anshim middle school. The only grade IV area is the commercial zone near Taegu Department 3) It is highly useful to evaluate urban biotope using the evaluation criteria constructed for this research, the evaluation criteria we developed may be difficult to guarantee the ability for reproduction and recovery, the appearance-number of the rare and endangered species are difficult to estimate urban biotopes. In the future, it is highly useful to evaluate biotope in terms of conservation of species and habitat through its valuation criteria developed in this research and it is necessary to develop the evaluation criteria applicable to estimation of biotope inventory and reproduction of biotope.